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RNA was isolated from transgenic plants from each independent transformation event and their corresponding control plants, and qRT-PCR was performed using primers (5 to 3) F1 (tcccaacatgtacggagcaa) and R1 (aactgctgcaatgcttccaa) and an internal probe (acactttttgtcaagatc)
I just simply rub it on with a rag and there ya go
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They were able to finance importation of laborers, acquire more land by headrights, grow more tobacco, and restart the cycle by financing importation of more laborers
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