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The treated cells were washed with cooled PBS, collected and mixed with 10% sulfosalicylic acid solution to remove proteins, and centrifuged at 13,000 g for 10 min at 4C

Relative gene expression levels were calculated using the Ct method, normalized to ACTB as the internal control

People with High Environmental Exposure If you live in a city with high pollution, work outdoors in the sun, or deal with high levels of daily stress, your glutathione stores are likely being depleted faster than average

A 10 L sperm suspension was examined on a microscope slide using a phase-contrast microscope (Olympus BX41, Tokyo, Japan) to confirm motility 41

These compounds are often obtained through unregulated sources that may lack quality control standards