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Cells were transfected with equal amounts of GCGR, GsS-, Gi1-, Gi2-, Gi3-, GoA-, GoB- or Gz-Rluc8, G3, and G1-, G8- or G9-GFP2 (as described in [21]), and pcDNA3.1() using polyethyleneimine (ESM Table 3)

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These reactions typically manifest as mild erythema (redness), pruritus (itching), swelling, or discomfort at the injection site

1 Introduction The idea that substances produced by duodenal mucosa could stimulate and initiate pancreatic endocrine, thereby reducing the amount of urinary glucose in patients with diabetes, was first proposed in 1906 (Moore, 1906)

331,332 In mesial temporal lobe epilepsy or tuberous sclerosis, astrocytes demonstrate a specific form of reactivity characterised by an increased expression of GFAP 333 and morphological atrophy manifested by reduced complexity of arbour and loss of distal leaflets